Dye Exchange in Micellar Solutions. Quantitative Analysis of Bulk and Single Molecule Fluorescence Titrations
نویسندگان
چکیده
A quantitative empirical model is presented, which relates the total surfactant concentration with the fluorescence intensity and the mean translational diffusion coefficient of dyes in micellar solutions, as determined by Fluorescence Correlation Spectroscopy (FCS). Based on this model a systematic data analysis method is defined for the precise determination of the dye binding equilibrium constant, the critical micelle concentration, the translational diffusion coefficients, and the hydrodynamic radii of dyes 10
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